BRP44L ELISA Kit

Catalog No. ABIN2043021

The Human BRP44L ELISA Kit (ABIN2043021) is a Colorimetric ELISA Kit designed to quantify Human BRP44L.

Quick Overview for BRP44L ELISA Kit (ABIN2043021)

Target: BRP44L (Brain Protein 44-Like (BRP44L))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.15-10 ng/mL

Application: ELISA

Product Details BRP44L ELISA Kit

Minimum Detection Limit: 0.15 ng/mL

Sensitivity: < 0.073 ng/mL

Application Details

Plate: Pre-coated

Protocol: Brain Protein 44 Like Protein(BRP44L) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Brain Protein 44 Like Protein(BRP44L). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Brain Protein 44 Like Protein(BRP44L). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Brain Protein 44 Like Protein(BRP44L), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Brain Protein 44 Like Protein(BRP44L) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for BRP44L

Target: BRP44L (Brain Protein 44-Like (BRP44L))

Alternative Name: Brain Protein 44 Like Protein(BRP44L)

Pathways: Warburg Effect