CAMK2B ELISA Kit

Catalog No. ABIN2043049

Human CAMK2B ELISA Kit Colorimetric assay for quantification of Human CAMK2B.

Quick Overview for CAMK2B ELISA Kit (ABIN2043049)

Target: CAMK2B (Calcium/calmodulin-Dependent Protein Kinase II beta (CAMK2B))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.31-20 ng/mL

Application: ELISA

Product Details CAMK2B ELISA Kit

Minimum Detection Limit: 0.31 ng/mL

Sensitivity: < 0.113 ng/mL

Application Details

Plate: Pre-coated

Protocol: Calcium/Calmodulin Dependent Protein Kinase II Beta (CAMK2b) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Calcium/Calmodulin Dependent Protein Kinase II Beta (CAMK2b). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Calcium/Calmodulin Dependent Protein Kinase II Beta (CAMK2b). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Calcium/Calmodulin Dependent Protein Kinase II Beta (CAMK2b), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Calcium/Calmodulin Dependent Protein Kinase II Beta (CAMK2b) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for CAMK2B

Target: CAMK2B (Calcium/calmodulin-Dependent Protein Kinase II beta (CAMK2B))

Alternative Name: Calcium/Calmodulin Dependent Protein Kinase II beta (CAMK2b)

Pathways: WNT Signaling, Interferon-gamma Pathway, Myometrial Relaxation and Contraction, Regulation of G-Protein Coupled Receptor Protein Signaling, Smooth Muscle Cell Migration, Regulation of long-term Neuronal Synaptic Plasticity