CAMKK2 ELISA Kit

Catalog No. ABIN2043051

The Human CAMKK2 ELISA Kit (ABIN2043051) is a Colorimetric ELISA Kit designed to quantify Human CAMKK2.

Quick Overview for CAMKK2 ELISA Kit (ABIN2043051)

Target: CAMKK2 (Calcium/calmodulin-Dependent Protein Kinase Kinase 2, beta (CAMKK2))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.31-20 ng/mL

Application: ELISA

Product Details CAMKK2 ELISA Kit

Minimum Detection Limit: 0.31 ng/mL

Sensitivity: < 0.114 ng/mL

Application Details

Plate: Pre-coated

Protocol: Calcium/Calmodulin Dependent Protein Kinase Kinase 2 (CAMKK2) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Calcium/Calmodulin Dependent Protein Kinase Kinase 2 (CAMKK2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Calcium/Calmodulin Dependent Protein Kinase Kinase 2 (CAMKK2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Calcium/Calmodulin Dependent Protein Kinase Kinase 2 (CAMKK2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Calcium/Calmodulin Dependent Protein Kinase Kinase 2 (CAMKK2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for CAMKK2

Target: CAMKK2 (Calcium/calmodulin-Dependent Protein Kinase Kinase 2, beta (CAMKK2))

Alternative Name: Calcium/Calmodulin Dependent Protein Kinase Kinase 2 (CAMKK2)

Pathways: AMPK Signaling