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CPZ ELISA Kit (Carboxypeptidase Z) ELISA Kit

CPZ Reactivity: Human Colorimetric Sandwich ELISA 0.62-40 ng/mL
Catalog No. ABIN2043069
$982.14
Plus shipping costs $45.00
96 tests
local_shipping Shipping to: United States
Delivery in 16 to 21 Business Days
  • Target
    Reactivity
    Human
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Detection Range
    0.62-40 ng/mL
    Minimum Detection Limit
    0.62 ng/mL
    Application
    ELISA
    Brand
    BioAssay™
    Sensitivity
    < 0.226 ng/mL
  • Target
  • Plate
    Pre-coated
    Protocol
    Carboxypeptidase Z (CPZ) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Carboxypeptidase Z (CPZ). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Carboxypeptidase Z (CPZ). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Carboxypeptidase Z (CPZ), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Carboxypeptidase Z (CPZ) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
    Restrictions
    For Research Use only
  • Storage
    4 °C/-20 °C
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