Cathelicidin ELISA Kit

Catalog No. ABIN2043085

Mouse Cathelicidin ELISA Kit, Colorimetric assay for quantification of Mouse Cathelicidin.

Quick Overview for Cathelicidin ELISA Kit (ABIN2043085)

Target: Cathelicidin (CAMP) (Cathelicidin Antimicrobial Peptide (CAMP))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.31-20 ng/mL

Application: ELISA

Product Details Cathelicidin ELISA Kit

Minimum Detection Limit: 0.31 ng/mL

Sensitivity: < 0.129 ng/mL

Application Details

Plate: Pre-coated

Protocol: Cathelicidin Antimicrobial Peptide (CAMP) BioAssay-ELISA Kit (Mouse) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cathelicidin Antimicrobial Peptide (CAMP). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cathelicidin Antimicrobial Peptide (CAMP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cathelicidin Antimicrobial Peptide (CAMP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Cathelicidin Antimicrobial Peptide (CAMP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for Cathelicidin

Target: Cathelicidin (CAMP) (Cathelicidin Antimicrobial Peptide (CAMP))

Pathways: Cellular Response to Molecule of Bacterial Origin