Cathepsin S ELISA Kit

Catalog No. ABIN2043093

This Rat Cathepsin S ELISA Kit is a Colorimetric ELISA Kit designed to quantify Rat Cathepsin S.

Quick Overview for Cathepsin S ELISA Kit (ABIN2043093)

Target: Cathepsin S (CTSS)

Reactivity: Rat

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.62-40 ng/mL

Application: ELISA

Product Details Cathepsin S ELISA Kit

Minimum Detection Limit: 0.62 ng/mL

Sensitivity: < 0.227 ng/mL

Application Details

Plate: Pre-coated

Protocol: Cathepsin S (CTSS) BioAssay-ELISA Kit (Rat) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Cathepsin S (CTSS). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Cathepsin S (CTSS). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Cathepsin S (CTSS), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Cathepsin S (CTSS) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for Cathepsin S

Target: Cathepsin S (CTSS)

Pathways: Activation of Innate immune Response, Toll-Like Receptors Cascades