SIVA1 ELISA Kit

Catalog No. ABIN2043101

This Colorimetric ELISA kit is designed for the quantitative measurement of Human SIVA1.

Quick Overview for SIVA1 ELISA Kit (ABIN2043101)

Target: SIVA1 (SIVA1, Apoptosis-Inducing Factor (SIVA1))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.15-10 ng/mL

Application: ELISA

Product Details SIVA1 ELISA Kit

Minimum Detection Limit: 0.15 ng/mL

Sensitivity: < 0.068 ng/mL

Application Details

Plate: Pre-coated

Protocol: CD27 Binding Protein (CD27BP) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to CD27 Binding Protein (CD27BP). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to CD27 Binding Protein (CD27BP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain CD27 Binding Protein (CD27BP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of CD27 Binding Protein (CD27BP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for SIVA1

Target: SIVA1 (SIVA1, Apoptosis-Inducing Factor (SIVA1))

Alternative Name: CD27 Binding Protein (CD27BP)

Pathways: Positive Regulation of Endopeptidase Activity