CENPE ELISA Kit
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- Target See all CENPE ELISA Kits
- CENPE (Centromere Protein E, 312kDa (CENPE))
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Reactivity
- Human
- Detection Method
- Colorimetric
- Method Type
- Sandwich ELISA
- Detection Range
- 0.31-20 ng/mL
- Minimum Detection Limit
- 0.31 ng/mL
- Application
- ELISA
- Brand
- BioAssay™
- Sensitivity
- < 0.124 ng/mL
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- Plate
- Pre-coated
- Protocol
- Centromere Protein E (CENPE) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Centromere Protein E (CENPE). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Centromere Protein E (CENPE). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Centromere Protein E (CENPE), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Centromere Protein E (CENPE) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- Restrictions
- For Research Use only
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- Storage
- 4 °C/-20 °C
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- Target See all CENPE ELISA Kits
- CENPE (Centromere Protein E, 312kDa (CENPE))
- Alternative Name
- Centromere Protein E (CENPE) (CENPE Products)
- Synonyms
- CENP-E ELISA Kit, KIF10 ELISA Kit, PPP1R61 ELISA Kit, 312kDa ELISA Kit, AU019344 ELISA Kit, BC049989 ELISA Kit, C530022J18 ELISA Kit, Kif10 ELISA Kit, CENPE ELISA Kit, kif10 ELISA Kit, cenp-E ELISA Kit, XCENP-E ELISA Kit, wu:fc06a10 ELISA Kit, zgc:152925 ELISA Kit, centromere protein E ELISA Kit, centromere protein E, 312kDa ELISA Kit, CENPE ELISA Kit, Cenpe ELISA Kit, cenpe ELISA Kit
- Pathways
- M Phase, Maintenance of Protein Location
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