Claudin 9 ELISA Kit

Catalog No. ABIN2043167

Product Details Claudin 9 ELISA Kit

Target: Claudin 9 (CLDN9)

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 78.12-5000 pg/mL

Minimum Detection Limit: 78.12 pg/mL

Application: ELISA

Brand: BioAssay™

Sensitivity: < 33 pg/mL

Application Details

Plate: Pre-coated

Protocol: Claudin 9 (CLDN9) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Claudin 9 (CLDN9). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Claudin 9 (CLDN9). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Claudin 9 (CLDN9), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Claudin 9 (CLDN9) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for Claudin 9

Target: Claudin 9 (CLDN9)

Abstract: CLDN9 Products

Synonyms: CLDN9 ELISA Kit, nmf329 ELISA Kit, claudin 9 ELISA Kit, CLDN9 ELISA Kit, Cldn9 ELISA Kit

Pathways: Cell-Cell Junction Organization, Hepatitis C