C1QA ELISA Kit

Catalog No. ABIN2043195

Product Details C1QA ELISA Kit

Target: C1QA (Complement Component 1, Q Subcomponent, A Chain (C1QA))

Reactivity: Rat

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 1.56-100 ng/mL

Minimum Detection Limit: 1.56 ng/mL

Application: ELISA

Brand: BioAssay™

Sensitivity: < 0.72 ng/mL

Application Details

Plate: Pre-coated

Protocol: Complement Component 1, Q Subcomponent A (C1qA) BioAssay-ELISA Kit (Rat) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Complement Component 1, Q Subcomponent A (C1qA). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Complement Component 1, Q Subcomponent A (C1qA). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Complement Component 1, Q Subcomponent A (C1qA), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Complement Component 1, Q Subcomponent A (C1qA) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for C1QA

Target: C1QA (Complement Component 1, Q Subcomponent, A Chain (C1QA))

Alternative Name: Complement Component 1, Q Subcomponent A (C1qA) (C1QA Products)

Synonyms: zgc:109903 ELISA Kit, C1QA ELISA Kit, AI255395 ELISA Kit, C1q ELISA Kit, complement C1q A chain ELISA Kit, complement component 1, q subcomponent, A chain ELISA Kit, complement component 1, q subcomponent, alpha polypeptide ELISA Kit, C1QA ELISA Kit, c1qa ELISA Kit, C1qa ELISA Kit

Pathways: Complement System