CFHR5 ELISA Kit

Catalog No. ABIN2043215

This Colorimetric ELISA kit is designed for the quantitative measurement of Human CFHR5.

Quick Overview for CFHR5 ELISA Kit (ABIN2043215)

Target: CFHR5 (Complement Factor H-Related 5 (CFHR5))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 1.56-100 ng/mL

Application: ELISA

Product Details CFHR5 ELISA Kit

Minimum Detection Limit: 1.56 ng/mL

Sensitivity: < 0.74 ng/mL

Application Details

Plate: Pre-coated

Protocol: Complement Factor H Related Protein 5 (CFHR5) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Complement Factor H Related Protein 5 (CFHR5). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Complement Factor H Related Protein 5 (CFHR5). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Complement Factor H Related Protein 5 (CFHR5), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Complement Factor H Related Protein 5 (CFHR5) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for CFHR5

Target: CFHR5 (Complement Factor H-Related 5 (CFHR5))

Alternative Name: Complement Factor H Related Protein 5 (CFHR5)