CUZD1 ELISA Kit

Catalog No. ABIN2043247

Human CUZD1 ELISA Kit, Colorimetric assay for quantification of Human CUZD1.

Quick Overview for CUZD1 ELISA Kit (ABIN2043247)

Target: CUZD1 (CUB and Zona Pellucida-Like Domains 1 (CUZD1))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.31-20 ng/mL

Application: ELISA

Product Details CUZD1 ELISA Kit

Minimum Detection Limit: 0.31 ng/mL

Sensitivity: < 0.114 ng/mL

Application Details

Plate: Pre-coated

Protocol: CUB And Zona Pellucida Like Domains Protein 1 (CUZD1) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to CUB And Zona Pellucida Like Domains Protein 1 (CUZD1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to CUB And Zona Pellucida Like Domains Protein 1 (CUZD1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain CUB And Zona Pellucida Like Domains Protein 1 (CUZD1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of CUB And Zona Pellucida Like Domains Protein 1 (CUZD1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for CUZD1

Target: CUZD1 (CUB and Zona Pellucida-Like Domains 1 (CUZD1))

Alternative Name: CUB And Zona Pellucida Like Domains Protein 1 (CUZD1)