DDX5 ELISA Kit

Catalog No. ABIN2043291

The Human DDX5 ELISA Kit (ABIN2043291) is a Colorimetric ELISA Kit designed to quantify Human DDX5.

Quick Overview for DDX5 ELISA Kit (ABIN2043291)

Target: DDX5 (DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 5 (DDX5))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.15-10 ng/mL

Application: ELISA

Product Details DDX5 ELISA Kit

Minimum Detection Limit: 0.15 ng/mL

Sensitivity: < 0.062 ng/mL

Application Details

Plate: Pre-coated

Protocol: DEAD Box Polypeptide 5 (DDX5) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to DEAD Box Polypeptide 5 (DDX5). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to DEAD Box Polypeptide 5 (DDX5). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain DEAD Box Polypeptide 5 (DDX5), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of DEAD Box Polypeptide 5 (DDX5) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for DDX5

Target: DDX5 (DEAD (Asp-Glu-Ala-Asp) Box Polypeptide 5 (DDX5))

Alternative Name: DEAD Box Polypeptide 5 (DDX5)

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Nuclear Hormone Receptor Binding, Regulation of Muscle Cell Differentiation, Positive Regulation of Response to DNA Damage Stimulus