DLL1 ELISA Kit

Catalog No. ABIN2043319

Rat DLL1 ELISA Kit, Colorimetric assay for quantification of Rat DLL1.

Quick Overview for DLL1 ELISA Kit (ABIN2043319)

Target: DLL1 (delta-Like 1 (DLL1))

Reactivity: Rat

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.78-50 ng/mL

Application: ELISA

Product Details DLL1 ELISA Kit

Minimum Detection Limit: 0.78 ng/mL

Sensitivity: < 0.30 ng/mL

Application Details

Plate: Pre-coated

Protocol: Delta Like 1 Homolog (dLK1) BioAssay-ELISA Kit (Rat) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Delta Like 1 Homolog (dLK1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Delta Like 1 Homolog (dLK1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Delta Like 1 Homolog (dLK1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Delta Like 1 Homolog (dLK1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for DLL1

Target: DLL1 (delta-Like 1 (DLL1))

Alternative Name: delta Like 1 Homolog (dLK1)

Pathways: Notch Signaling, Stem Cell Maintenance