DSTYK ELISA Kit

Catalog No. ABIN2043369

The Rat DSTYK ELISA Kit (ABIN2043369) is a Colorimetric ELISA Kit designed to quantify Rat DSTYK.

Quick Overview for DSTYK ELISA Kit (ABIN2043369)

Target: DSTYK (Dual serine/threonine and tyrosine Protein Kinase (DSTYK))

Reactivity: Rat

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.31-20 ng/mL

Application: ELISA

Product Details DSTYK ELISA Kit

Minimum Detection Limit: 0.31 ng/mL

Sensitivity: < 0.113 ng/mL

Application Details

Plate: Pre-coated

Protocol: Dual Serine/Threonine And Tyrosine Protein Kinase (DSTYK) BioAssay-ELISA Kit (Rat) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dual Serine/Threonine And Tyrosine Protein Kinase (DSTYK). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Dual Serine/Threonine And Tyrosine Protein Kinase (DSTYK). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Dual Serine/Threonine And Tyrosine Protein Kinase (DSTYK), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Dual Serine/Threonine And Tyrosine Protein Kinase (DSTYK) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for DSTYK

Target: DSTYK (Dual serine/threonine and tyrosine Protein Kinase (DSTYK))