DNHD1 ELISA Kit
Quick Overview for DNHD1 ELISA Kit (ABIN2043371)
Target
See all DNHD1 productsReactivity
Detection Method
Method Type
Detection Range
Application
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Minimum Detection Limit
- 0.31 ng/mL
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Sensitivity
- < 0.112 ng/mL
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Plate
- Pre-coated
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Protocol
- Dynein Heavy Chain Domain Containing Protein 1 (DNHD1) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Dynein Heavy Chain Domain Containing Protein 1 (DNHD1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Dynein Heavy Chain Domain Containing Protein 1 (DNHD1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Dynein Heavy Chain Domain Containing Protein 1 (DNHD1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Dynein Heavy Chain Domain Containing Protein 1 (DNHD1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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Restrictions
- For Research Use only
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Storage
- 4 °C/-20 °C
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- DNHD1 (Dynein Heavy Chain Domain 1 (DNHD1))
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Alternative Name
- Dynein Heavy Chain Domain Containing Protein 1 (DNHD1)
Target See all DNHD1 products
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