EML2 ELISA Kit

Catalog No. ABIN2043375

This Rat EML2 ELISA Kit is a Colorimetric ELISA Kit designed to quantify Rat EML2.

Quick Overview for EML2 ELISA Kit (ABIN2043375)

Target: EML2 (Echinoderm Microtubule Associated Protein Like 2 (EML2))

Reactivity: Rat

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.31-20 ng/mL

Application: ELISA

Product Details EML2 ELISA Kit

Minimum Detection Limit: 0.31 ng/mL

Sensitivity: < 0.126 ng/mL

Application Details

Plate: Pre-coated

Protocol: Echinoderm Microtubule Associated Protein Like Protein 2 (EML2) BioAssay-ELISA Kit (Rat) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Echinoderm Microtubule Associated Protein Like Protein 2 (EML2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Echinoderm Microtubule Associated Protein Like Protein 2 (EML2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Echinoderm Microtubule Associated Protein Like Protein 2 (EML2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Echinoderm Microtubule Associated Protein Like Protein 2 (EML2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for EML2

Target: EML2 (Echinoderm Microtubule Associated Protein Like 2 (EML2))

Alternative Name: Echinoderm Microtubule Associated Protein Like Protein 2 (EML2)

Pathways: Microtubule Dynamics, Sensory Perception of Sound