EGFL6 ELISA Kit

Catalog No. ABIN2043377

Product Details EGFL6 ELISA Kit

Target: EGFL6 (EGF-Like-Domain, Multiple 6 (EGFL6))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 93.75-6000 pg/mL

Minimum Detection Limit: 93.75 pg/mL

Application: ELISA

Brand: BioAssay™

Sensitivity: < 47.25 pg/mL

Application Details

Plate: Pre-coated

Protocol: EGF Like Domain Protein, Multiple 6 (EGFL6) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to EGF Like Domain Protein, Multiple 6 (EGFL6). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to EGF Like Domain Protein, Multiple 6 (EGFL6). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain EGF Like Domain Protein, Multiple 6 (EGFL6), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of EGF Like Domain Protein, Multiple 6 (EGFL6) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for EGFL6

Target: EGFL6 (EGF-Like-Domain, Multiple 6 (EGFL6))

Alternative Name: EGF Like Domain Protein, Multiple 6 (EGFL6) (EGFL6 Products)