EDIL3 ELISA Kit

Catalog No. ABIN2043385

Human EDIL3 ELISA Kit, Colorimetric assay for quantification of Human EDIL3.

Quick Overview for EDIL3 ELISA Kit (ABIN2043385)

Target: EDIL3 (DEL1) (EGF like repeats and discoidin domains 3 (DEL1))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.31-20 ng/mL

Application: ELISA

Product Details EDIL3 ELISA Kit

Minimum Detection Limit: 0.31 ng/mL

Sensitivity: < 0.131 ng/mL

Application Details

Plate: Pre-coated

Protocol: EGF Like Repeats And Discoidin I Like Domains Protein 3 (EDIL3) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to EGF Like Repeats And Discoidin I Like Domains Protein 3 (EDIL3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to EGF Like Repeats And Discoidin I Like Domains Protein 3 (EDIL3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain EGF Like Repeats And Discoidin I Like Domains Protein 3 (EDIL3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of EGF Like Repeats And Discoidin I Like Domains Protein 3 (EDIL3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for EDIL3

Target: EDIL3 (DEL1) (EGF like repeats and discoidin domains 3 (DEL1))

Alternative Name: EGF Like Repeats And Discoidin I Like Domains Protein 3 (EDIL3)