ELOVL1 ELISA Kit

Catalog No. ABIN2043397

Rat ELOVL1 ELISA Kit, Colorimetric assay for quantification of Rat ELOVL1.

Quick Overview for ELOVL1 ELISA Kit (ABIN2043397)

Target: ELOVL1 (ELOVL Fatty Acid Elongase 1 (ELOVL1))

Reactivity: Rat

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.15-10 ng/mL

Application: ELISA

Product Details ELOVL1 ELISA Kit

Minimum Detection Limit: 0.15 ng/mL

Sensitivity: < 0.054 ng/mL

Application Details

Plate: Pre-coated

Protocol: Elongation Of Very Long Chain Fatty Acids Like Protein 1 (ELOVL1) BioAssay-ELISA Kit (Rat) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Elongation Of Very Long Chain Fatty Acids Like Protein 1 (ELOVL1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Elongation Of Very Long Chain Fatty Acids Like Protein 1 (ELOVL1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Elongation Of Very Long Chain Fatty Acids Like Protein 1 (ELOVL1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Elongation Of Very Long Chain Fatty Acids Like Protein 1 (ELOVL1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for ELOVL1

Target: ELOVL1 (ELOVL Fatty Acid Elongase 1 (ELOVL1))

Alternative Name: Elongation Of Very Long Chain Fatty Acids Like Protein 1 (ELOVL1)