Epigen ELISA Kit

Catalog No. ABIN2043407

This Colorimetric ELISA kit is designed for the quantitative measurement of Human Epigen.

Quick Overview for Epigen ELISA Kit (ABIN2043407)

Target: Epigen (EPGN) (Epithelial Mitogen (EPGN))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.31-20 ng/mL

Application: ELISA

Product Details Epigen ELISA Kit

Minimum Detection Limit: 0.31 ng/mL

Brand: BioAssay™

Sensitivity: < 0.112 ng/mL

Application Details

Plate: Pre-coated

Protocol: Epigen (EPG) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Epigen (EPG). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Epigen (EPG). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Epigen (EPG), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Epigen (EPG) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for Epigen

Target: Epigen (EPGN) (Epithelial Mitogen (EPGN))

Alternative Name: Epigen (EPG)

Pathways: RTK Signaling, EGFR Signaling Pathway