FAM135B ELISA Kit

Catalog No. ABIN2043433

Mouse FAM135B ELISA Kit, Colorimetric assay for quantification of Mouse FAM135B.

Quick Overview for FAM135B ELISA Kit (ABIN2043433)

Target: FAM135B (Family with Sequence Similarity 135, Member B (FAM135B))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.15-10 ng/mL

Application: ELISA

Product Details FAM135B ELISA Kit

Minimum Detection Limit: 0.15 ng/mL

Sensitivity: < 0.057 ng/mL

Application Details

Plate: Pre-coated

Protocol: Family With Sequence Similarity 135, Member B (FAM135B) BioAssay-ELISA Kit (Mouse) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Family With Sequence Similarity 135, Member B (FAM135B). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Family With Sequence Similarity 135, Member B (FAM135B). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Family With Sequence Similarity 135, Member B (FAM135B), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Family With Sequence Similarity 135, Member B (FAM135B) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for FAM135B

Target: FAM135B (Family with Sequence Similarity 135, Member B (FAM135B))