FGF21 ELISA Kit

Catalog No. ABIN2043473

This Colorimetric ELISA kit is designed for the quantitative measurement of Mouse FGF21.

Quick Overview for FGF21 ELISA Kit (ABIN2043473)

Target: FGF21 (Fibroblast Growth Factor 21 (FGF21))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 7.81-500 pg/mL

Application: ELISA

Product Details FGF21 ELISA Kit

Minimum Detection Limit: 7.81 pg/mL

Sensitivity: < 3.2 pg/mL

Application Details

Plate: Pre-coated

Protocol: Fibroblast Growth Factor 21 (FGF21) BioAssay-ELISA Kit (Mouse) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Fibroblast Growth Factor 21 (FGF21). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Fibroblast Growth Factor 21 (FGF21). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Fibroblast Growth Factor 21 (FGF21), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Fibroblast Growth Factor 21 (FGF21) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for FGF21

Target: FGF21 (Fibroblast Growth Factor 21 (FGF21))

Pathways: RTK Signaling