FBLN3 ELISA Kit

Catalog No. ABIN2043483

The Mouse FBLN3 ELISA Kit (ABIN2043483) is a Colorimetric ELISA Kit designed to quantify Mouse FBLN3.

Quick Overview for FBLN3 ELISA Kit (ABIN2043483)

Target: FBLN3 (Fibulin 3 (FBLN3))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 15.62-1000 pg/mL

Application: ELISA

Product Details FBLN3 ELISA Kit

Minimum Detection Limit: 15.62 pg/mL

Sensitivity: < 6.6 pg/mL

Application Details

Plate: Pre-coated

Protocol: Fibulin 3 (FBLN3) BioAssay-ELISA Kit (Mouse) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Fibulin 3 (FBLN3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Fibulin 3 (FBLN3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Fibulin 3 (FBLN3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Fibulin 3 (FBLN3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for FBLN3

Target: FBLN3 (Fibulin 3 (FBLN3))

Pathways: EGFR Signaling Pathway