Frataxin ELISA Kit

Catalog No. ABIN2043505

Product Details Frataxin ELISA Kit

Target: Frataxin (FXN)

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.15-10 ng/mL

Minimum Detection Limit: 0.15 ng/mL

Application: ELISA

Brand: BioAssay™

Sensitivity: < 0.055 ng/mL

Application Details

Plate: Pre-coated

Protocol: Frataxin (FXN) BioAssay-ELISA Kit (Mouse) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Frataxin (FXN). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Frataxin (FXN). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Frataxin (FXN), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Frataxin (FXN) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for Frataxin

Target: Frataxin (FXN)

Abstract: FXN Products

Synonyms: zgc:158218 ELISA Kit, LOC100284365 ELISA Kit, BcDNA:AT09528 ELISA Kit, CG8971 ELISA Kit, DFH ELISA Kit, Dfh ELISA Kit, Dmel\\CG8971 ELISA Kit, X25 ELISA Kit, dfh ELISA Kit, CyaY ELISA Kit, FA ELISA Kit, FARR ELISA Kit, FRDA ELISA Kit, RGD1565754 ELISA Kit, Frda ELISA Kit, frataxin ELISA Kit, fxn ELISA Kit, LOC100284365 ELISA Kit, TVAG_182150 ELISA Kit, fh ELISA Kit, FTX1 ELISA Kit, FXN ELISA Kit, Fxn ELISA Kit

Pathways: Transition Metal Ion Homeostasis, Regulation of Cell Size