GSAP ELISA Kit
Quick Overview for GSAP ELISA Kit (ABIN2043535)
Target
See all GSAP (PION) ELISA KitsReactivity
Detection Method
Method Type
Detection Range
Application
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Minimum Detection Limit
- 0.31 ng/mL
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Sensitivity
- < 0.113 ng/mL
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Pigeon Homolog (PION) ELISA Kit
PION Reactivity: Human Colorimetric Sandwich ELISA 1.563 ng/mL - 100 ng/mL Plasma, Serum, Tissue Homogenate
Pigeon Homolog (PION) ELISA KitPION Reactivity: Rat Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Tissue Homogenate
Pigeon Homolog (PION) ELISA KitPION Reactivity: Human Colorimetric Sandwich ELISA 1.563 ng/mL - 100 ng/mL Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate
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Plate
- Pre-coated
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Protocol
- Gamma-Secretase Activating Protein (gSAP) BioAssay-ELISA Kit (Rat) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Gamma-Secretase Activating Protein (gSAP). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Gamma-Secretase Activating Protein (gSAP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Gamma-Secretase Activating Protein (gSAP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Gamma-Secretase Activating Protein (gSAP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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Restrictions
- For Research Use only
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Storage
- 4 °C/-20 °C
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- GSAP (PION) (Pigeon Homolog (PION))
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Alternative Name
- gamma-Secretase Activating Protein (gSAP)
Target See all GSAP (PION) ELISA Kits
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