GLP-2 ELISA Kit

Catalog No. ABIN2043547

Pig GLP-2 ELISA Kit, Colorimetric assay for quantification of Pig GLP-2.

Quick Overview for GLP-2 ELISA Kit (ABIN2043547)

Target: GLP-2 (Glucagon-like peptide 2 (GLP-2))

Reactivity: Pig

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 123.46-10000 pg/mL

Application: ELISA

Product Details GLP-2 ELISA Kit

Minimum Detection Limit: 123.46 pg/mL

Sensitivity: < 43.8 pg/mL

Application Details

Plate: Pre-coated

Protocol: Glucagon Like Peptide 2 (GLP2) BioAssay-ELISA Kit (Porcine) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Glucagon Like Peptide 2 (GLP2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Glucagon Like Peptide 2 (GLP2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Glucagon Like Peptide 2 (GLP2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Glucagon Like Peptide 2 (GLP2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for GLP-2

Target: GLP-2 (Glucagon-like peptide 2 (GLP-2))

Alternative Name: Glucagon Like Peptide 2 (GLP2)