Glutaredoxin 1 ELISA Kit

Catalog No. ABIN2043567

This Mouse Glutaredoxin 1 ELISA Kit is a Colorimetric ELISA Kit designed to quantify Mouse Glutaredoxin 1.

Quick Overview for Glutaredoxin 1 ELISA Kit (ABIN2043567)

Target: Glutaredoxin 1 (GRX1)

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 78.12-5000 pg/mL

Application: ELISA

Product Details Glutaredoxin 1 ELISA Kit

Minimum Detection Limit: 78.12 pg/mL

Sensitivity: < 32 pg/mL

Application Details

Plate: Pre-coated

Protocol: Glutaredoxin (GLRX) BioAssay-ELISA Kit (Mouse) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Glutaredoxin (GLRX). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Glutaredoxin (GLRX). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Glutaredoxin (GLRX), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Glutaredoxin (GLRX) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for Glutaredoxin 1

Target: Glutaredoxin 1 (GRX1)

Alternative Name: Glutaredoxin (GLRX)

Target Type: Viral Protein

Pathways: Cell RedoxHomeostasis