Glutaredoxin 1 ELISA Kit

Catalog No. ABIN6951137

Product Details Glutaredoxin 1 ELISA Kit

Target: Glutaredoxin 1 (GRX1)

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Purpose: Human GLRX ELISA Kit.

Sample Type: Cell Culture Supernatant, Cell Samples, Plasma, Serum, Tissue Lysate

Analytical Method: Quantitative

Characteristics:

• Strip plates and additional reagents allow for use in multiple experiments
• Quantitative protein detection
• Establishes normal range
• The best products for confirmation of antibody array data

Components:

• Pre-Coated 96-well Strip Microplate
• Wash Buffer
• Stop Solution
• Assay Diluent(s)
• Lyophilized Standard
• Biotinylated Detection Antibody
• Streptavidin-Conjugated HRP
• TMB One-Step Substrate

Material not included:

• Distilled or deionized water
• Precision pipettes to deliver 2 µl to 1 µl volumes
• Adjustable 1-25 µl pipettes for reagent preparation
• 100 µl and 1 liter graduated cylinders
• Tubes to prepare standard and sample dilutions
• Absorbent paper
• Microplate reader capable of measuring absorbance at 450nm
• Log-log graph paper or computer and software for ELISA data analysis

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Plate: Pre-coated

Protocol:

1. Prepare all reagents, samples and standards as instructed in the manual.
2. Add 100 µl of standard or sample to each well.
3. Incubate 2.5 h at RT or O/N at 4°C.
4. Add 100 µl of prepared biotin antibody to each well.
5. Incubate 1 h at RT.
6. Add 100 µl of prepared Streptavidin solution to each well.
7. Incubate 45 min at RT.
8. Add 100 µl of TMB One-Step Substrate Reagent to each well.
9. Incubate 30 min at RT.
10. Add 50 µl of Stop Solution to each well.
11. Read at 450 nm immediately.

Restrictions: For Research Use only

Handling

Expiry Date: 6 months

Target Details for Glutaredoxin 1

Target: Glutaredoxin 1 (GRX1)

Alternative Name: GLRX (GRX1 Products)

Synonyms: Glrx1 ELISA Kit, Grx ELISA Kit, grx ELISA Kit, wu:fc38f02 ELISA Kit, zgc:103707 ELISA Kit, GLRXL ELISA Kit, Grx1 ELISA Kit, TTase ELISA Kit, C86710 ELISA Kit, D13Wsu156e ELISA Kit, grx1 ELISA Kit, GLRX ELISA Kit, GRX ELISA Kit, GRX1 ELISA Kit, GLRX1 ELISA Kit, TTF ELISA Kit, glrx ELISA Kit, glutaredoxin ELISA Kit, glutaredoxin (thioltransferase) ELISA Kit, glutaredoxin Grx1 ELISA Kit, NrdH-redoxin ELISA Kit, Uncharacterized monothiol glutaredoxin F10D7.3 ELISA Kit, glutaredoxin-1 (Grx1) ELISA Kit, temporal expression: late ELISA Kit, glutaredoxin L homeolog ELISA Kit, Glrx ELISA Kit, glrx ELISA Kit, GLRX ELISA Kit, grx1 ELISA Kit, AF_RS07750 ELISA Kit, F10D7.3 ELISA Kit, AFUA_1G06100 ELISA Kit, NT01EI_2528 ELISA Kit, O2L ELISA Kit, glrx.L ELISA Kit

Target Type: Viral Protein

Gene ID: 2745

UniProt: P35754

Pathways: Cell RedoxHomeostasis