GM2A ELISA Kit

Catalog No. ABIN2043589

Human GM2A ELISA Kit, Colorimetric assay for quantification of Human GM2A.

Quick Overview for GM2A ELISA Kit (ABIN2043589)

Target: GM2A (GM2 Ganglioside Activator (GM2A))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.15-10 ng/mL

Application: ELISA

Product Details GM2A ELISA Kit

Minimum Detection Limit: 0.15 ng/mL

Sensitivity: < 0.059 ng/mL

Application Details

Plate: Pre-coated

Protocol: GM2 Ganglioside Activator (GM2A) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to GM2 Ganglioside Activator (GM2A). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to GM2 Ganglioside Activator (GM2A). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain GM2 Ganglioside Activator (GM2A), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of GM2 Ganglioside Activator (GM2A) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for GM2A

Target: GM2A (GM2 Ganglioside Activator (GM2A))