GADD45G ELISA Kit

Catalog No. ABIN2043601

Mouse GADD45G ELISA Kit Colorimetric assay for quantification of Mouse GADD45G.

Quick Overview for GADD45G ELISA Kit (ABIN2043601)

Target: GADD45G (Growth Arrest and DNA-Damage-Inducible, gamma (GADD45G))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.62-40 ng/mL

Application: ELISA

Product Details GADD45G ELISA Kit

Minimum Detection Limit: 0.62 ng/mL

Sensitivity: < 0.225 ng/mL

Application Details

Plate: Pre-coated

Protocol: Growth Arrest And DNA Damage Inducible Protein Gamma (GADD45g) BioAssay-ELISA Kit (Mouse) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Growth Arrest And DNA Damage Inducible Protein Gamma (GADD45g). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Growth Arrest And DNA Damage Inducible Protein Gamma (GADD45g). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Growth Arrest And DNA Damage Inducible Protein Gamma (GADD45g), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Growth Arrest And DNA Damage Inducible Protein Gamma (GADD45g) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for GADD45G

Target: GADD45G (Growth Arrest and DNA-Damage-Inducible, gamma (GADD45G))

Alternative Name: Growth Arrest And DNA Damage Inducible Protein gamma (GADD45g)

Pathways: Cell Division Cycle