HSPA12B ELISA Kit

Catalog No. ABIN2043609

The Human HSPA12B ELISA Kit (ABIN2043609) is a Colorimetric ELISA Kit designed to quantify Human HSPA12B.

Quick Overview for HSPA12B ELISA Kit (ABIN2043609)

Target: HSPA12B (Heat Shock 70kD Protein 12B (HSPA12B))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.31-20 ng/mL

Application: ELISA

Product Details HSPA12B ELISA Kit

Minimum Detection Limit: 0.31 ng/mL

Sensitivity: < 0.128 ng/mL

Application Details

Plate: Pre-coated

Protocol: Heat Shock 70kDa Protein 12B (HSPA12B) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Heat Shock 70kDa Protein 12B (HSPA12B). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Heat Shock 70kDa Protein 12B (HSPA12B). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Heat Shock 70kDa Protein 12B (HSPA12B), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Heat Shock 70kDa Protein 12B (HSPA12B) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for HSPA12B

Target: HSPA12B (Heat Shock 70kD Protein 12B (HSPA12B))

Alternative Name: Heat Shock 70kDa Protein 12B (HSPA12B)