HSP90 ELISA Kit
Quick Overview for HSP90 ELISA Kit (ABIN2043617)
Target
See all HSP90 ELISA KitsReactivity
Detection Method
Method Type
Detection Range
Application
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Minimum Detection Limit
- 3.12 ng/mL
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Brand
- BioAssay™
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Sensitivity
- < 1.28 ng/mL
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Components
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A: Microtiter Plate, 1x96 wells, Pre-coated, ready to use
B: Standard, 2x1vial
C: Standard Diluent, 1x20 mL
D: Detection Reagent A, 1x120 µL
E: Detection Reagent B, 1x120 µL
F: Assay Diluent A, 1x10 mL
G: Assay Diluent B, 1x10 mL
H: TMB Substrate, 1x0 mL
K: Stop Solution, 1x0 mL
L: Wash Buffer, 30x, 1x20 mL
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Heat Shock Protein 90 (HSP90) ELISA Kit
HSP90 Reactivity: Human Colorimetric Sandwich ELISA 3.12 ng/mL - 200 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Heat Shock Protein 90 (HSP90) ELISA KitHSP90 Reactivity: Rat Colorimetric Sandwich ELISA 125 pg/mL - 8000 pg/mL Cell Culture Supernatant, Plasma, Serum
Heat Shock Protein 90 (HSP90) ELISA KitHSP90 Reactivity: Mouse Colorimetric Sandwich ELISA 0.16 ng/mL - 10 ng/mL Cell Culture Supernatant, Plasma, Serum
Heat Shock Protein 90 (HSP90) ELISA KitHSP90 Reactivity: Rat Colorimetric Sandwich ELISA 125 pg/mL - 8000 pg/mL Plasma, Serum, Tissue Homogenate
Heat Shock Protein 90 (HSP90) ELISA KitHSP90 Reactivity: Mouse Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Heat Shock Protein 90 (HSP90) ELISA KitHigh Sensitivity HSP90 Reactivity: Human Colorimetric Sandwich ELISA 78 pg/mL - 5000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Heat Shock Protein 90 (HSP90) ELISA KitHSP90 Reactivity: Cow Colorimetric Sandwich ELISA 3.12 ng/mL - 200 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
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Plate
- Pre-coated
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Protocol
- The Human Heat Shock Protein 90 (HSP90) ELISA Kit is a sandwich enzyme immunoassay for the in vitro quantitative measurement of HSP90 in human serum, plasma, tissue homogenates, cell lysates, cell culture supernatants and other biological fluids.The microtiter plate provided in this kit has been pre-coated with an antibody specific to HSP90. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to HSP90. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain HSP90, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulfuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of HSP90 in the sample is then determined by comparing the O.D. of the sample to the standard curve.
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Restrictions
- For Research Use only
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Storage
- 4 °C/-20 °C
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- HSP90 (Heat Shock Protein 90 (HSP90))
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Pathways
- M Phase, Regulation of Cell Size, Signaling Events mediated by VEGFR1 and VEGFR2, VEGFR1 Specific Signals
Target See all HSP90 ELISA Kits
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