HDLBP ELISA Kit
Quick Overview for HDLBP ELISA Kit (ABIN2043645)
Target
See all HDLBP ELISA KitsReactivity
Detection Method
Method Type
Detection Range
Application
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Minimum Detection Limit
- 0.15 ng/mL
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Sensitivity
- < 0.056 ng/mL
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High Density Lipoprotein Binding Protein (HDLBP) ELISA Kit
HDLBP Reactivity: Human Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Culture Supernatant, Cell Lysate, Tissue Homogenate
High Density Lipoprotein Binding Protein (HDLBP) ELISA KitHDLBP Reactivity: Human Colorimetric Sandwich ELISA 1.563 ng/mL - 100 ng/mL Plasma, Serum, Tissue Homogenate
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Plate
- Pre-coated
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Protocol
- High Density Lipoprotein Binding Protein (HDLBP) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to High Density Lipoprotein Binding Protein (HDLBP). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to High Density Lipoprotein Binding Protein (HDLBP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain High Density Lipoprotein Binding Protein (HDLBP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of High Density Lipoprotein Binding Protein (HDLBP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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Restrictions
- For Research Use only
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Storage
- 4 °C/-20 °C
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- HDLBP (High Density Lipoprotein Binding Protein (HDLBP))
Target See all HDLBP ELISA Kits
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