IMPDH2 ELISA Kit

Catalog No. ABIN2043745

Human IMPDH2 ELISA Kit Colorimetric assay for quantification of Human IMPDH2.

Quick Overview for IMPDH2 ELISA Kit (ABIN2043745)

Target: IMPDH2 (IMP (Inosine 5'-Monophosphate) Dehydrogenase 2 (IMPDH2))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.78-50 ng/mL

Application: ELISA

Product Details IMPDH2 ELISA Kit

Minimum Detection Limit: 0.78 ng/mL

Sensitivity: < 0.27 ng/mL

Application Details

Plate: Pre-coated

Protocol: Inosine 5'-Monophosphate Dehydrogenase 2 (IMPDH2) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Inosine 5'-Monophosphate Dehydrogenase 2 (IMPDH2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Inosine 5'-Monophosphate Dehydrogenase 2 (IMPDH2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Inosine 5'-Monophosphate Dehydrogenase 2 (IMPDH2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Inosine 5'-Monophosphate Dehydrogenase 2 (IMPDH2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for IMPDH2

Target: IMPDH2 (IMP (Inosine 5'-Monophosphate) Dehydrogenase 2 (IMPDH2))

Alternative Name: Inosine 5'-Monophosphate Dehydrogenase 2 (IMPDH2)

Pathways: Ribonucleoside Biosynthetic Process, SARS-CoV-2 Protein Interactome