IL12B ELISA Kit

Catalog No. ABIN2043801

This Pig IL12B ELISA Kit is a Colorimetric ELISA Kit designed to quantify Pig IL12B.

Quick Overview for IL12B ELISA Kit (ABIN2043801)

Target: IL12B (Interleukin 12 beta (IL12B))

Reactivity: Pig

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 7.81-500 pg/mL

Application: ELISA

Product Details IL12B ELISA Kit

Minimum Detection Limit: 7.81 pg/mL

Sensitivity: < 2.8 pg/mL

Application Details

Plate: Pre-coated

Protocol: Interleukin 12B (IL12B) BioAssay-ELISA Kit (Porcine) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interleukin 12B (IL12B). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Interleukin 12B (IL12B). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Interleukin 12B (IL12B), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Interleukin 12B (IL12B) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for IL12B

Target: IL12B (Interleukin 12 beta (IL12B))

Alternative Name: Interleukin 12B (IL12B)

Pathways: JAK-STAT Signaling, Cellular Response to Molecule of Bacterial Origin, Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process, Activated T Cell Proliferation