IL-7 ELISA Kit

Catalog No. ABIN2043817

Mouse IL-7 ELISA Kit Colorimetric assay for quantification of Mouse IL-7.

Quick Overview for IL-7 ELISA Kit (ABIN2043817)

Target: IL-7 (IL7) (Interleukin 7 (IL7))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 15.62-1000 pg/mL

Application: ELISA

Product Details IL-7 ELISA Kit

Minimum Detection Limit: 15.62 pg/mL

Sensitivity: < 5.1 pg/mL

Application Details

Plate: Pre-coated

Protocol: Interleukin 7 (IL7) BioAssay-ELISA Kit (Mouse) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Interleukin 7 (IL7). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Interleukin 7 (IL7). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Interleukin 7 (IL7), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Interleukin 7 (IL7) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for IL-7

Target: IL-7 (IL7) (Interleukin 7 (IL7))

Pathways: JAK-STAT Signaling