Cytokeratin 18 ELISA Kit

Catalog No. ABIN2043851

This Colorimetric ELISA kit is designed for the quantitative measurement of Pig Cytokeratin 18.

Quick Overview for Cytokeratin 18 ELISA Kit (ABIN2043851)

Target: Cytokeratin 18 (KRT18) (Keratin 18 (KRT18))

Reactivity: Pig

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.15-10 ng/mL

Application: ELISA

Product Details Cytokeratin 18 ELISA Kit

Minimum Detection Limit: 0.15 ng/mL

Sensitivity: < 0.054 ng/mL

Application Details

Plate: Pre-coated

Protocol: Keratin 18 (KRT18) BioAssay-ELISA Kit (Porcine) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Keratin 18 (KRT18). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Keratin 18 (KRT18). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Keratin 18 (KRT18), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Keratin 18 (KRT18) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for Cytokeratin 18

Target: Cytokeratin 18 (KRT18) (Keratin 18 (KRT18))

Pathways: Apoptosis, Caspase Cascade in Apoptosis