Keratin 33A ELISA Kit
The Human Keratin 33A ELISA Kit (ABIN2043855) is a Colorimetric ELISA Kit designed to quantify Human Keratin 33A.
Catalog No. ABIN2043855
$1,255.14
Plus shipping costs $50.00
96 tests
Shipping to:
United States
Delivery in 16 to 22 Business Days
Quick Overview for Keratin 33A ELISA Kit (ABIN2043855)
Target
See all Keratin 33A (KRT33A) products
Keratin 33A (KRT33A)
Reactivity
Human
Detection Method
Colorimetric
Method Type
Sandwich ELISA
Detection Range
78.12-5000 pg/mL
Application
ELISA
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Minimum Detection Limit
- 78.12 pg/mL
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Sensitivity
- < 29 pg/mL
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Keratin 33A (KRT33A) ELISA Kit
KRT33A Reactivity: Human Colorimetric Sandwich ELISA 78 pg/mL - 5000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
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Plate
- Pre-coated
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Protocol
- Keratin 33A (KRT33A) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Keratin 33A (KRT33A). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Keratin 33A (KRT33A). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Keratin 33A (KRT33A), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Keratin 33A (KRT33A) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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Restrictions
- For Research Use only
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Storage
- 4 °C/-20 °C
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- Keratin 33A (KRT33A)
Target See all Keratin 33A (KRT33A) products
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