KRT81 ELISA Kit

Catalog No. ABIN2043859

Product Details KRT81 ELISA Kit

Target: KRT81 (Keratin 81 (KRT81))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.15-10 ng/mL

Minimum Detection Limit: 0.15 ng/mL

Application: ELISA

Brand: BioAssay™

Sensitivity: < 0.053 ng/mL

Application Details

Plate: Pre-coated

Protocol: Keratin 81 (KRT81) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Keratin 81 (KRT81). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Keratin 81 (KRT81). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Keratin 81 (KRT81), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Keratin 81 (KRT81) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for KRT81

Target: KRT81 (Keratin 81 (KRT81))

Abstract: KRT81 Products

Synonyms: KRT81 ELISA Kit, KRTHB5 ELISA Kit, HB1 ELISA Kit, Hb-1 ELISA Kit, KRTHB1 ELISA Kit, MLN137 ELISA Kit, ghHkb1 ELISA Kit, hHAKB2-1 ELISA Kit, AA589625 ELISA Kit, Krt2-19 ELISA Kit, keratin 81 ELISA Kit, keratin, type II cuticular Hb1 ELISA Kit, keratin 85 ELISA Kit, KRT81 ELISA Kit, LOC100012075 ELISA Kit, LOC100092864 ELISA Kit, LOC100394162 ELISA Kit, LOC100481102 ELISA Kit, LOC100526784 ELISA Kit, KRT85 ELISA Kit, Krt81 ELISA Kit