WWC1 ELISA Kit

Catalog No. ABIN2043861

The Mouse WWC1 ELISA Kit (ABIN2043861) is a Colorimetric ELISA Kit designed to quantify Mouse WWC1.

Quick Overview for WWC1 ELISA Kit (ABIN2043861)

Target: WWC1 (WW and C2 Domain Containing 1 (WWC1))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 78.12-5000 pg/mL

Application: ELISA

Product Details WWC1 ELISA Kit

Minimum Detection Limit: 78.12 pg/mL

Sensitivity: < 30 pg/mL

Application Details

Plate: Pre-coated

Protocol: Kidney And Brain Protein (KIBRA) BioAssay-ELISA Kit (Mouse) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Kidney And Brain Protein (KIBRA). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Kidney And Brain Protein (KIBRA). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Kidney And Brain Protein (KIBRA), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Kidney And Brain Protein (KIBRA) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for WWC1

Target: WWC1 (WW and C2 Domain Containing 1 (WWC1))

Alternative Name: KIBRA (Kidney And Brain Protein)