Lamin B2 ELISA Kit

Catalog No. ABIN2043871

Human Lamin B2 ELISA Kit, Colorimetric assay for quantification of Human Lamin B2.

Quick Overview for Lamin B2 ELISA Kit (ABIN2043871)

Target: Lamin B2 (LMNB2)

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.15-10 ng/mL

Application: ELISA

Product Details Lamin B2 ELISA Kit

Minimum Detection Limit: 0.15 ng/mL

Sensitivity: < 0.051 ng/mL

Application Details

Plate: Pre-coated

Protocol: Lamin B2 (LMNB2) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Lamin B2 (LMNB2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Lamin B2 (LMNB2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Lamin B2 (LMNB2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Lamin B2 (LMNB2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for Lamin B2

Target: Lamin B2 (LMNB2)

Pathways: Apoptosis, Caspase Cascade in Apoptosis