LRRC3C ELISA Kit
Quick Overview for LRRC3C ELISA Kit (ABIN2043879)
Target
See all LRRC3C productsReactivity
Detection Method
Method Type
Detection Range
Application
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Minimum Detection Limit
- 0.15 ng/mL
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Sensitivity
- < 0.066 ng/mL
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Plate
- Pre-coated
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Protocol
- Leucine Rich Repeat Containing Protein 3C (LRRC3C) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Leucine Rich Repeat Containing Protein 3C (LRRC3C). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Leucine Rich Repeat Containing Protein 3C (LRRC3C). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Leucine Rich Repeat Containing Protein 3C (LRRC3C), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Leucine Rich Repeat Containing Protein 3C (LRRC3C) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
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Restrictions
- For Research Use only
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Storage
- 4 °C/-20 °C
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- LRRC3C (Leucine Rich Repeat Containing 3C (LRRC3C))
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Alternative Name
- Leucine Rich Repeat Containing Protein 3C (LRRC3C)
Target See all LRRC3C products
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