LRRC4B ELISA Kit

Catalog No. ABIN2043883

Human LRRC4B ELISA Kit, Colorimetric assay for quantification of Human LRRC4B.

Quick Overview for LRRC4B ELISA Kit (ABIN2043883)

Target: LRRC4B (Leucine Rich Repeat Containing 4B (LRRC4B))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.31-20 ng/mL

Application: ELISA

Product Details LRRC4B ELISA Kit

Minimum Detection Limit: 0.31 ng/mL

Sensitivity: < 0.133 ng/mL

Application Details

Plate: Pre-coated

Protocol: Leucine Rich Repeat Containing Protein 4B (LRRC4B) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Leucine Rich Repeat Containing Protein 4B (LRRC4B). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Leucine Rich Repeat Containing Protein 4B (LRRC4B). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Leucine Rich Repeat Containing Protein 4B (LRRC4B), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Leucine Rich Repeat Containing Protein 4B (LRRC4B) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for LRRC4B

Target: LRRC4B (Leucine Rich Repeat Containing 4B (LRRC4B))

Alternative Name: Leucine Rich Repeat Containing Protein 4B (LRRC4B)

Pathways: Synaptic Membrane