Lipase A ELISA Kit

Catalog No. ABIN2043893

This Colorimetric ELISA kit is designed for the quantitative measurement of Mouse Lipase A.

Quick Overview for Lipase A ELISA Kit (ABIN2043893)

Target: Lipase A (LIPA) (Lipase A, Lysosomal Acid, Cholesterol Esterase (LIPA))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.31-20 ng/mL

Application: ELISA

Product Details Lipase A ELISA Kit

Minimum Detection Limit: 0.31 ng/mL

Sensitivity: < 0.117 ng/mL

Application Details

Plate: Pre-coated

Protocol: Lipase A, Lysosomal Acid (LIPA) BioAssay-ELISA Kit (Mouse) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Lipase A, Lysosomal Acid (LIPA). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Lipase A, Lysosomal Acid (LIPA). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Lipase A, Lysosomal Acid (LIPA), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Lipase A, Lysosomal Acid (LIPA) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for Lipase A

Target: Lipase A (LIPA) (Lipase A, Lysosomal Acid, Cholesterol Esterase (LIPA))

Alternative Name: Lipase A, Lysosomal Acid (LIPA)