MAT2A ELISA Kit

Catalog No. ABIN2043967

Rat MAT2A ELISA Kit, Colorimetric assay for quantification of Rat MAT2A.

Quick Overview for MAT2A ELISA Kit (ABIN2043967)

Target: MAT2A (Methionine Adenosyltransferase II, alpha (MAT2A))

Reactivity: Rat

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.15-10 ng/mL

Application: ELISA

Product Details MAT2A ELISA Kit

Minimum Detection Limit: 0.15 ng/mL

Sensitivity: < 0.054 ng/mL

Application Details

Plate: Pre-coated

Protocol: Methionine Adenosyltransferase II Alpha (MAT2a) BioAssay-ELISA Kit (Rat) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Methionine Adenosyltransferase II Alpha (MAT2a). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Methionine Adenosyltransferase II Alpha (MAT2a). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Methionine Adenosyltransferase II Alpha (MAT2a), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Methionine Adenosyltransferase II Alpha (MAT2a) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for MAT2A

Target: MAT2A (Methionine Adenosyltransferase II, alpha (MAT2A))

Alternative Name: Methionine Adenosyltransferase II alpha (MAT2a)

Pathways: Ribonucleoside Biosynthetic Process, Methionine Biosynthetic Process