MID1 ELISA Kit (Midline 1) ELISA Kit
MID1
Reactivity: Mouse
Colorimetric
Sandwich ELISA
0.15-10 ng/mL
Catalog No. ABIN2043975
$999.43
Plus shipping costs $45.00
96 tests
local_shipping
Shipping to:
United States
Delivery in 16 to 21 Business Days
-
- Target
- Reactivity
- Mouse
- Detection Method
- Colorimetric
- Method Type
- Sandwich ELISA
- Detection Range
- 0.15-10 ng/mL
- Minimum Detection Limit
- 0.15 ng/mL
- Application
- ELISA
- Brand
- BioAssay™
- Sensitivity
- < 0.052 ng/mL
-
- Target
-
- Plate
- Pre-coated
- Protocol
- Midline 1 (MID1) BioAssay-ELISA Kit (Mouse) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Midline 1 (MID1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Midline 1 (MID1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Midline 1 (MID1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Midline 1 (MID1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
- Restrictions
- For Research Use only
-
- Storage
- 4 °C/-20 °C
-
You are here:
