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MID1 ELISA Kit (Midline 1) ELISA Kit

MID1 Reactivity: Mouse Colorimetric Sandwich ELISA 0.15-10 ng/mL
Catalog No. ABIN2043975
$999.43
Plus shipping costs $45.00
96 tests
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Delivery in 16 to 21 Business Days
  • Target
    Reactivity
    Mouse
    Detection Method
    Colorimetric
    Method Type
    Sandwich ELISA
    Detection Range
    0.15-10 ng/mL
    Minimum Detection Limit
    0.15 ng/mL
    Application
    ELISA
    Brand
    BioAssay™
    Sensitivity
    < 0.052 ng/mL
  • Target
  • Plate
    Pre-coated
    Protocol
    Midline 1 (MID1) BioAssay-ELISA Kit (Mouse) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Midline 1 (MID1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Midline 1 (MID1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Midline 1 (MID1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Midline 1 (MID1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
    Restrictions
    For Research Use only
  • Storage
    4 °C/-20 °C
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