MTFMT ELISA Kit

Catalog No. ABIN2043981

This Mouse MTFMT ELISA Kit is a Colorimetric ELISA Kit designed to quantify Mouse MTFMT.

Quick Overview for MTFMT ELISA Kit (ABIN2043981)

Target: MTFMT (Mitochondrial Methionyl-tRNA Formyltransferase (MTFMT))

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.15-10 ng/mL

Application: ELISA

Product Details MTFMT ELISA Kit

Minimum Detection Limit: 0.15 ng/mL

Sensitivity: < 0.055 ng/mL

Application Details

Plate: Pre-coated

Protocol: Mitochondrial Methionyl tRNA Formyltransferase (MTFMT) BioAssay-ELISA Kit (Mouse) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mitochondrial Methionyl tRNA Formyltransferase (MTFMT). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Mitochondrial Methionyl tRNA Formyltransferase (MTFMT). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mitochondrial Methionyl tRNA Formyltransferase (MTFMT), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Mitochondrial Methionyl tRNA Formyltransferase (MTFMT) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for MTFMT

Target: MTFMT (Mitochondrial Methionyl-tRNA Formyltransferase (MTFMT))

Alternative Name: Mitochondrial Methionyl tRNA Formyltransferase (MTFMT)