MAPK12 ELISA Kit

Catalog No. ABIN2043983

This Colorimetric ELISA kit is designed for the quantitative measurement of Human MAPK12.

Quick Overview for MAPK12 ELISA Kit (ABIN2043983)

Target: MAPK12 (Mitogen-Activated Protein Kinase 12 (MAPK12))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.15-10 ng/mL

Application: ELISA

Product Details MAPK12 ELISA Kit

Minimum Detection Limit: 0.15 ng/mL

Sensitivity: < 0.072 ng/mL

Application Details

Plate: Pre-coated

Protocol: Mitogen Activated Protein Kinase 12 (MAPK12) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mitogen Activated Protein Kinase 12 (MAPK12). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Mitogen Activated Protein Kinase 12 (MAPK12). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mitogen Activated Protein Kinase 12 (MAPK12), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Mitogen Activated Protein Kinase 12 (MAPK12) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for MAPK12

Target: MAPK12 (Mitogen-Activated Protein Kinase 12 (MAPK12))

Alternative Name: Mitogen Activated Protein Kinase 12 (MAPK12)

Pathways: MAPK Signaling, Neurotrophin Signaling Pathway, Regulation of Muscle Cell Differentiation, Hepatitis C, BCR Signaling, S100 Proteins