MUC12 ELISA Kit

Catalog No. ABIN2043991

Human MUC12 ELISA Kit, Colorimetric assay for quantification of Human MUC12.

Quick Overview for MUC12 ELISA Kit (ABIN2043991)

Target: MUC12 (Mucin 12, Cell Surface Associated (MUC12))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 0.31-20 ng/mL

Application: ELISA

Product Details MUC12 ELISA Kit

Minimum Detection Limit: 0.31 ng/mL

Sensitivity: < 0.132 ng/mL

Application Details

Plate: Pre-coated

Protocol: Mucin 12, Cell Surface Associated (MUC12) BioAssay-ELISA Kit (Human) is a Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mucin 12, Cell Surface Associated (MUC12). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Mucin 12, Cell Surface Associated (MUC12). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mucin 12, Cell Surface Associated (MUC12), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm±10nm. The concentration of Mucin 12, Cell Surface Associated (MUC12) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Restrictions: For Research Use only

Handling

Storage: 4 °C/-20 °C

Target Details for MUC12

Target: MUC12 (Mucin 12, Cell Surface Associated (MUC12))